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Densonucleosis Virus DNA: Analysis of Fine Structure by Electron Microscopy and Agarose Gel Electrophoresis

N.E.R.C., Unit of Invertebrate Virology, 5 South Parks Road, Oxford OX1 3UB

Double stranded DNA, created on extraction of the plus and minus strands, which are separately packaged in densonucleosis virus particles, exists predominantly as linear monomers, although circular monomers and concatamers and other less well defined structures were observed by electron microscopy and agarose gel electrophoresis. A limited nucleotide sequence permutation, which is probably non-random, comprises about 2.7% of the genome (160 base pairs) and is considered to be the structural feature causing circularization and concatamerization. Evidence of an inverted terminal repetition observed by circularization of single stranded DNA was obtained by electron microscopy and S₁ nuclease digestion. Estimates of the size of the terminal repeats varied from 60 to 380 base pairs.

Received 12 September 1977; accepted 10 January 1978.