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Localization of Simian Adenovirus 7 (SA 7) Transcription and Replication in Lytic Infection. An Ultracyto-chemical and Autoradiographical Study

Institut de Recherches Scientifiques sur le Cancer, B.P. no 8, F-94800 Villejuif, France

We have studied SA7 (simian adenovirus 7) lytic infection at the ultrastructural level. The use of cytochemical techniques which specifically stain DNA or preferentially stain ribonucleoproteins permitted the analysis of the structure of the virus-induced nuclear inclusions, and revealed presumed virus DNA before the appearance of other nuclear alterations. Correlation of these findings with high resolution autoradiography enabled the functions of virus DNA replication and transcription to be ascribed to a defined nuclear inclusion. We demonstrate that the nucleolus remains distinct from the inclusion body, contrary to the situation in other adenovirus-infected cells. The functional role of host cell chromatin and of the nucleolus are discussed.

Received 25 November 1977; accepted 20 January 1978.