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Complement-mediated Cytolysis of HSV-1 and HSV-2 Infected Cells: Plasma Membrane Antigens Reactive with Type-specific and Cross-reactive Antibody

Lawrence A. Wilson

Terry W. Fenger

Jerry W. Smith^,

^* Unit for Laboratory Animal Medicine, and Department of Microbiology, University of Michigan School of Medicine, Ann Arbor, Michigan 48109, U.S.A.
Department of Microbiology, Louisiana State University Medical Center, 1542 Tulane Avenue, New Orleans, Louisiana 70112, U.S.A.

Surface antigens of BHK-21 cells infected with HSV-1 or HSV-2 were radio-iodinated (¹²⁵I) with lactoperoxidase, immune precipitated and analysed by polyacrylamide gel electrophoresis (PAGE). Experiments using antiserum to HSV-1 or HSV-2, absorbed with appropriate homotypic or heterotypic antigens, revealed that both type-specific (homotypic) and cross-reactive antibody combined with surface glycoproteins to form a single large radioactive peak. This peak, which constituted the major glycoprotein region (region a) observed in electropherograms, represented a range in mol. wt. from 115000 to 130000. Sensitization of cells to complement lysis, neutralization of infectious virus and immune precipitation of surface glycoproteins (region a) were found to be generally correlated properties of all the antibody preparations analysed, including antibody prepared specifically against region a antigens. These findings suggest a major immunological role for the surface glycoproteins migrating in PAGE region a.

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Received 15 December 1977; accepted 7 March 1978.