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J Gen Virol 40 (1978), 465-469; DOI 10.1099/0022-1317-40-2-465
© 1978 Society for General Microbiology
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The Use of the Enzyme Linked Immunosorbent Assay to Detect a Nuclear Polyhedrosis Virus in Heliothis armigera Larvae

D. C. Kelly, Mary-L. Edwards, H. F. Evans and J. S. Robertson

N.E.R.C., Unit of Invertebrate Virology, 5 South Parks Road, Oxford, OX1 3UB, U.K.

The enzyme linked immunosorbent assay (ELISA) has been shown to be a specific and sensitive method to detect a nuclear polyhedrosis virus in Heliothis armigera larvae. 1 ng/ml of purified virus particle antigen could be detected. The presence of larval extracts did not interfere in the assay. The growth of the virus in Heliothis armigera larvae was analysed by ELISA and the conventional counting of virus polyhedra and this showed that ELISA was a superior detection method.

Received 23 January 1978; accepted 22 February 1978.




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Copyright © 1978 by the Society for General Microbiology.