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In Vitro Infectivity of the Autographa californica Nuclear Polyhedrosis Virus Under Various Conditions

Boyce Thompson Institute, Yonkers, New York 10701, U.S.A.

The stability of the in vitro infectivity of the non-occluded virions (NOV) of the Autographa californica nuclear polyhedrosis virus (Ac-NPV) was tested. Exposure of Ac-NPV NOV to solutions ranging from pH 7.5 to 11.4 with molarities of 0.1 to 0.001 showed that infectivity was most stable in 0.001 M, pH 7.5, tris(hydroxymethyl)aminomethane (tris)-HCl buffer. Infectious NOV could be concentrated by precipitation with 50% saturated ammonium sulphate or 9% (w/w) polyethylene glycol 4000. Treatment of concentrated preparations with 1 M-urea did not affect infectivity or the centrifugal properties of the NOV. NOV were partially purified by centrifugation through a sucrose underlay.

Received 9 January 1978; accepted 22 March 1978.