HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Tamura, T.-A. Articles by Takano, T. Search for Related Content PubMed Articles by Tamura, T.-A. Articles by Takano, T. Agricola Articles by Tamura, T.-A. Articles by Takano, T.

A New, Rapid Procedure for the Concentration of C-type Viruses from Large Quantities of Culture Media: Ultrafiltration by Diaflo Membrane and Purification by Ficoll Gradient Centrifugation

Department of Microbiology, Keio University School of Medicine, Shinjuku-ku, Tokyo, Japan-160

The Amicon TCIR recycle system with Diaflo PM30 filters provided a rapid, simple and inexpensive method for the concentration of baboon endogenous virus from a large quantity of culture medium from chronically infected cells. The recovery of infectivity and virion RNA-dependent DNA polymerase activity was substantially better than after the conventional concentration methods of ultra-centrifugation or precipitation with ammonium sulphate or polyethylene glycol 6000. The combination of ultrafiltration and Ficoll gradient centrifugation is recommended as a general procedure for the purification of fragile C-type viruses.

Received 2 February 1978; accepted 15 May 1978.