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J Gen Virol 41 (1978), 27-36; DOI 10.1099/0022-1317-41-1-27
© 1978 Society for General Microbiology
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Quantification of Cell Fusion by Twenty-one Strains of Newcastle Disease Virus Using Flow Microfluorometry

L. S. Cram, J. C. Forslund and J. H. Jett

Biophysics and Instrumentation Group, Los Alamos Scientific Laboratory, University of California, Los Alamos, New Mexico 87545, U.S.A.

The cell-fusing ability of Newcastle disease virus (NDV) was quantified using flow microfluorometry (FMF). The rate of polykaryocyte formation, fusion dependence on multiplicity of infection, and cell fusion differences for 21 NDV strains were measured using this technique. No correlation was found between the virulence of a virus strain and its cell fusion index calculated from the FMF data.

Received 6 October 1977; accepted 2 May 1978.




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Copyright © 1978 by the Society for General Microbiology.