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Effect of Herpes Simplex Virus Type 1 Infection on the Cellular DNA Polymerase Activities of Mouse Cell Cultures

Hygiene-Institut der Philipps-Universität, Pilgrimstein 2, D-355 Marburg, Federal Republic of Germany

Thymidine kinase-deficient mouse cell cultures infected with herpes simplex virus type 1 exhibited a maximum of virus DNA synthesis around 8 h post-infection as determined by pulse labelling with ³H-thymidine. Cellular DNA synthesis was progressively inhibited, but still appreciable until 8 h post-infection and not completely abolished at any time during the infectious cycle. Phosphonoacetic acid was found to be a potent and selective inhibitor of virus DNA synthesis only when added to infected cultures before the onset of virus DNA synthesis. During the interval of increasing virus DNA synthesis the activity of cellular polymerase decreased rapidly, whereas the polymerase activity increased significantly; a slight increase was observed for the polymerase activity. When infected cells were kept in the presence of phosphonoacetic acid following virus adsorption the effect on cellular DNA polymerases was less pronounced.

Received 16 February 1978; accepted 16 June 1978.