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Rapid Purification of Lymphocytic Choriomeningitis Virus by Density Gradient Centrifugation in Colloidal Silica

Hygiene-Institut der Phillipps-Universität, 3550 Marburg/Lahn Federal Republic of Germany

Lymphocytic choriomeningitis virus was purified from cell culture fluid by density gradient centrifugation in colloidal silica. The specific infectivity increased 5000-fold and the recovery of infectivity was about 10%.

^* Present address: Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie an der Universität Hamburg, Martinistrasse 52, 2000 Hamburg 20, Federal Republic of Germany.

Received 6 July 1978; accepted 21 August 1978.

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