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Purification and Some Properties of Two Viruses Infecting the Cultivated Mushroom Agaricus bisporus

Virology Department, Glasshouse Crops Research Institute, Worthing Road, Littlehampton, Sussex BN16 3PU, U.K.

Highly purified preparations of the isometric mushroom viruses 1 and 4 were made by a combination of differential centrifugation, ion-exchange chromatography on DEAE-cellulose and sucrose density-gradient centrifugation. Purified preparations of MV1 virions (25 nm diam.) sedimented at 130S contained one capsid polypeptide of mol. wt. 24400 and two species of double-stranded RNA of mol. wt. approx. 1.4 x 10⁶. MV4 particles (35 nm diam.) had a sedimentation value of 140 to 145S, contained one capsid polypeptide of mol. wt. 63800 and two species of dsRNA of mol. wt. 1.5 x 10⁶ and 1.4 x 10⁶. MV1 and MV4 were antigenically unrelated.

Received 21 July 1978; accepted 1 August 1978.