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J Gen Virol 43 (1979), 513-519; DOI 10.1099/0022-1317-43-3-513
© 1979 Society for General Microbiology
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A Plaque Assay for the Simian Rotavirus SA11

Eric M. Smith*, Mary Kolb Estes*,{dagger},, David Y. Graham{ddagger} and Charles P. Gerba*,{ddagger}

* Department of Virology and Epidemiology
{dagger} Department of Medicine, Baylor College of Medicine, Houston, Texas 77030, U.S.A.

A sensitive, quantitative and reproducible plaque assay for the measurement of the simian rotavirus SA11 is described. Plaque formation required the presence of the facilitators pancreatin or trypsin and diethylaminoethyl-dextran in the agar overlay. SA11 produced plaques in three continuous primate cell lines: MA-104, CV-1 and LLC-MK2. MA-104 cells were the most sensitive.

{ddagger} To whom reprint requests should be addressed.

Received 7 September 1978; accepted 24 November 1978.


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