HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Erickson, J. S. Articles by Paucker, K. Search for Related Content PubMed PubMed Citation Articles by Erickson, J. S. Articles by Paucker, K. Agricola Articles by Erickson, J. S. Articles by Paucker, K.

Molecular Species of Interferon Induced in Mouse L Cells by Newcastle Disease Virus and Polyriboinosinic-polyribocytidylic Acid

Microbiology Department, The Medical College of Pennsylvania, Philadelphia, Pennsylvania 19129, U.S.A.

Interferons were stimulated in mouse L cells by Newcastle disease virus (NDV) or by polyriboinosinic-polyribocytidylic acid poly(rI). poly(rC). These were fractionated by sequential affinity chromatography on bovine plasma albumin (BPA)-Sepharose and on -carboxypentyl (CH)-Sepharose. Based on their interaction with CH-Sepharose, interferor induced by NDV was resolved into three major bands of activity (L/NDV-1,2,3) and poly(rI). Poly(rC)-interferon into two (L/rI:rC-1,2). These interferon components were purified to a specific activity of 3 x 10⁷ to 4 x 10⁷ units/mg protein by antibody affinity chromatography and examined by electrophoresis in SDS-polyacrylamide gels. A total of five molecular species was thus identified for NDV-induced interferon and three for poly(rI). poly(rC) induced interferon, as summarized in Table 1. We conclude from our observations that mouse interferons can be produced by L cells in multiple forms with specific physiochemical properties and in proportions determined by the type of agent employed for induction.

Received 8 June 1978; accepted 30 November 1978.