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The Sensitivity of Measles Virus Haemolysin to Acetone and the Preparation of Mono-specific Human Anti-Haemolysin by Absorption

Department of Microbiology and Immunobiology The Queen's University of Belfast, Grosvenor Road, Belfast, BT12 6BN Northern Ireland

The haemolysin of measles virus, either in the virion or in infected cells, is functionally and antigenically sensitive to acetone. Absorption of human sera with acetone-treated, measles virus-infected cells removes antibodies to all measles virus structural antigens except haemolysin. The antibody titres of absorbed sera give good correlation in HLI, neutralization and fluorescent antibody staining on unfixed infected cells.

Received 7 February 1979; accepted 13 March 1979.