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J Gen Virol 44 (1979), 599-608; DOI 10.1099/0022-1317-44-3-599
© 1979 Society for General Microbiology

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Characterization of Influenza Virus RNA Transcripts Synthesized in vitro

A. J. Hay and J. J. Skehel

Division of Virology, National Institute for Medical Research, Mill Hill, London, NW7 U.K.

Polyadenylated transcripts synthesized in vitro by detergent-disrupted influenza virus resemble virus mRNAs in that they possess the complement of the 3' terminus of the genome RNAs but lack sequences corresponding to the same 5' terminal region, including the homologous sequence of nucleotides 1 to 22. Transcription is initiated at the 3' terminus by both ApG and GpG as well as in the absence of added primer.

Received 20 December 1978; accepted 14 February 1979.


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D. C. Pritlove, L. L. M. Poon, E. Fodor, J. Sharps, and G. G. Brownlee
Polyadenylation of Influenza Virus mRNA Transcribed In Vitro from Model Virion RNA Templates: Requirement for 5' Conserved Sequences
J. Virol., February 1, 1998; 72(2): 1280 - 1286.
[Abstract] [Full Text] [PDF]




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