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Genetic Analysis of Adenovirus Type 2. IX. The Physical Locations of Structural Genes

John A. Hassell

Département de Microbiologie, Centre Hospitalier Universitaire, Sherbrooke, Québec, Canada JIH 5N4 and Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, U.S.A.

Several virus-specified proteins of adenovirus type 2 (Ad2) and type 5 (Ad5) can be distinguished by their differential electrophoretic mobility in SDS-polyacrylamide gels. By comparing the physical maps of the genomes of interserotypic recombinants between ts mutants of these viruses with the pattern of proteins expressed, the physical locations of some genes can be determined on the adenovirus DNA molecule. The proteins of a total of 103 recombinants were analysed; 44 of these are of known DNA structure. Structural genes for the proteins analysed span the following loci on the conventional adenovirus map: V (30 to 43); III (43 to 44); II and 66K (55 to 57); 100K, 36K, 32K (69 to 71); IV (80 to 90).

^* Author to whom reprint requests should be addressed.

Present address: Department of Microbiology and Immunology, McGill University, Montreal, Canada.

Received 31 July 1978; accepted 22 February 1979.