HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Bem, F. Articles by Murant, A. F. Search for Related Content PubMed Articles by Bem, F. Articles by Murant, A. F. Agricola Articles by Bem, F. Articles by Murant, A. F.

Comparison of Particle Properties of Heracleum Latent and Apple Chlorotic Leaf Spot Viruses

A. F. Murant

Scottish Horticultural Research Institute, Invergowrie, Dundee, Scotland

Purified preparations of heracleum latent virus (HLV), which has very flexuous filamentous particles of about 730 x 12 nm, contain a single sedimenting component with s⁰_20,w = 96 S, A₂₆₀/A₂₈₀ = 1.50 and buoyant density in Cs₂SO₄ = 1.24 g/ml. The particles possess a single polypeptide, mol. wt. 23500, apparently lacking in tryptophan, and a single-stranded RNA of apparent mol. wt. 2.3 x 10⁶ in nondenaturing conditions or 2.15 x 10⁶ in denaturing conditions. The infectivity of the particles is sensitive to ribonuclease; this sensitivity is increased after exposure to EDTA, but is not decreased by addition of Mg²⁺ or Ca²⁺. HLV thus closely resembles apple chlorotic leaf spot virus, to which it is serologically unrelated; it is regarded as a tentative member of the closterovirus group. Its cryptogram is R/1:2.3/(5):E/E:S/Ve/Ap.

^* Present address: Benaki Phytopathological Institute, Kiphissia, Athens, Greece.

To whom requests for reprints should be addressed.

Received 19 January 1979; accepted 23 March 1979.