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Biophysical Properties of a Non-cultivable 29-nm Enteric Virus

Virology Division Special Viral Diagnostic Branch Center for Disease Control, Atlanta, Georgia 30333, U.S.A.

A 29 nm non-cultivable virus (NCV) was detected in faecal extracts from children hospitalized for gastroenteritis. The NCV had a density of 1.35 g/ml in glycerol-potassium tartrate density gradients and was resistant to degradation by proteolytic enzymes, non-ionic detergents and pH extremes. The surface of these virus particles had knob-like projections which appeared to have a symmetrical arrangement. When heated to 56 °C, the virus was completely degraded to soluble components which could not be seen by electron microscopy.

Received 6 December 1978; accepted 23 April 1979.