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J Gen Virol 44 (1979), 843-847; DOI 10.1099/0022-1317-44-3-843
© 1979 Society for General Microbiology

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Transformation and T Antigen Induction by Linearized BK Virus DNA

J. van der Noordaa, W. De Jong, W. Pauw, C. J. A. Sol and A. van Strien

Laboratorium voor de Gezondheidsleer University of Amsterdam, Mauritskade 57 1092 AD Amsterdam The Netherlands

Linearized unit length DNA obtained after cleavage of the supercoiled DNA of the human papovavirus BKV by PstI (0.31) induced transformation and T antigen less efficiently than DNA cleaved by EcoRI (0.0), BamHI (0.98), KpnI (0.90) or HhaI (0.73). BKV DNA cleaved by XbaI (0.27 and 0.95) did not induce T antigen.

Received 7 September 1978; accepted 3 May 1979.





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