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Analysis of the Products of Mixed Infection of Tobacco Protoplasts with Two Strains of Cowpea Chlorotic Mottle Virus

John Innes Institute, Colney Lane, Norwich NR4 7UH, U.K.

The progeny from mixed infections of tobacco protoplasts with a temperature sensitive (ts) and a temperature resistant (wt) strain of cowpea chlorotic mottle virus at restrictive temperatures (35 °C) was largely (90%) ts, judged by the coat protein produced by single lesion isolates grown at 25 °C. However, although apparently ts at 25 °C, many isolates were temperature resistant at 35 °C and produced virus. Successive ‘purification’ through single lesions allowed normal ts or wt viruses to be recovered from these unusual isolates. The genomes of ts and wt appear able to co-exist in mixed infections; ts is dominant over wt at all temperatures so that most of the progeny has ts genome, but wt remains available to rescue ts under restrictive conditions. There was no evidence that the phenomenon was due to recombination.

Received 29 January 1979; accepted 9 April 1979.