| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Medical Biophysics Branch, Whiteshell Nuclear Research Establishment, Atomic Energy of Canada Limited, Pinawa, Manitoba, Canada, R0E 1LO
Evidence is presented supporting the hypothesis that reovirus intermediate subviral particles (ISVP), which show increased infectivity relative to intact virions, can gain entry into host L cells by two alternative pathways. One pathway is by the process of viropexis, involving phagocytic vacuoles. A second entry pathway is via direct penetration of the plasma membrane of the cell, without involvement of a phagocytic vacuole. Using electron microscopy, a kinetic analysis of the uptake process was carried out. Results indicate that at 37 °C ISVP gain entry into host cells primarily by direct entry, although viropexis also occurs, while intact virions gain entry by viropexis almost exclusively. A second line of experimental evidence consistent with the idea that ISVP can ‘melt’ their way through the plasma membrane is provided by studies on the release of pre-loaded radioactive 51Cr from host cells following infection. 51Cr release data demonstrate that infection with ISVP leads to an immediate increased leakiness of the cell plasma membrane, whereas no such increase takes place following infection with an equivalent number of intact virions. This demonstrates that ISVP can interact with the plasma membrane of the cell in a manner which is qualitatively different from the interaction between intact virions and the plasma membrane.
The ability of ISVP to directly penetrate the plasma membrane of the host cell, which intact virions apparently cannot do, could explain the decreased duration of the eclipse phase, as well as the increased infectivity of ISVP, relative to that observed for infection with intact virions.
Received 3 July 1978;
accepted 17 April 1979.
This article has been cited by other articles:
|
|
 |
|
  E. M. Johnson, J. D. Doyle, J. D. Wetzel, R. P. McClung, N. Katunuma, J. D. Chappell, M. K. Washington, and T. S. Dermody Genetic and Pharmacologic Alteration of Cathepsin Expression Influences Reovirus Pathogenesis J. Virol., October 1, 2009; 83(19): 9630 - 9640. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Maginnis, B. A. Mainou, A. Derdowski, E. M. Johnson, R. Zent, and T. S. Dermody NPXY Motifs in the {beta}1 Integrin Cytoplasmic Tail Are Required for Functional Reovirus Entry J. Virol., April 1, 2008; 82(7): 3181 - 3191. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Maginnis, J. C. Forrest, S. A. Kopecky-Bromberg, S. K. Dickeson, S. A. Santoro, M. M. Zutter, G. R. Nemerow, J. M. Bergelson, and T. S. Dermody {beta}1 Integrin Mediates Internalization of Mammalian Reovirus J. Virol., March 15, 2006; 80(6): 2760 - 2770. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Danthi, M. W. Hansberger, J. A. Campbell, J. C. Forrest, and T. S. Dermody JAM-A-Independent, Antibody-Mediated Uptake of Reovirus into Cells Leads to Apoptosis J. Virol., February 1, 2006; 80(3): 1261 - 1270. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. E. VanCompernolle, R. J. Taylor, K. Oswald-Richter, J. Jiang, B. E. Youree, J. H. Bowie, M. J. Tyler, J. M. Conlon, D. Wade, C. Aiken, et al. Antimicrobial Peptides from Amphibian Skin Potently Inhibit Human Immunodeficiency Virus Infection and Transfer of Virus from Dendritic Cells to T Cells J. Virol., September 15, 2005; 79(18): 11598 - 11606. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Pal, C. S. Kaetzel, K. Brundage, C. A. Cunningham, and C. F. Cuff Regulation of polymeric immunoglobulin receptor expression by reovirus J. Gen. Virol., August 1, 2005; 86(8): 2347 - 2357. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Odegard, K. Chandran, X. Zhang, J. S. L. Parker, T. S. Baker, and M. L. Nibert Putative Autocleavage of Outer Capsid Protein {micro}1, Allowing Release of Myristoylated Peptide {micro}1N during Particle Uncoating, Is Critical for Cell Entry by Reovirus J. Virol., August 15, 2004; 78(16): 8732 - 8745. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. W. Golden, J. A. Bahe, W. T. Lucas, M. L. Nibert, and L. A. Schiff Cathepsin S Supports Acid-independent Infection by Some Reoviruses J. Biol. Chem., March 5, 2004; 279(10): 8547 - 8557. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. H. Ebert, S. A. Kopecky-Bromberg, and T. S. Dermody Cathepsin B Is Inhibited in Mutant Cells Selected during Persistent Reovirus Infection J. Biol. Chem., January 30, 2004; 279(5): 3837 - 3851. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Chandran, J. S. L. Parker, M. Ehrlich, T. Kirchhausen, and M. L. Nibert The {delta} Region of Outer-Capsid Protein {micro}1 Undergoes Conformational Change and Release from Reovirus Particles during Cell Entry J. Virol., December 15, 2003; 77(24): 13361 - 13375. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Becker, T. R. Peters, and T. S. Dermody Reovirus {sigma}NS and {micro}NS Proteins Form Cytoplasmic Inclusion Structures in the Absence of Viral Infection J. Virol., May 15, 2003; 77(10): 5948 - 5963. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. J. Wilson, E. L. Nason, C. S. Hardy, D. H. Ebert, J. D. Wetzel, B. V. Venkataram Prasad, and T. S. Dermody A Single Mutation in the Carboxy Terminus of Reovirus Outer-Capsid Protein {sigma}3 Confers Enhanced Kinetics of {sigma}3 Proteolysis, Resistance to Inhibitors of Viral Disassembly, and Alterations in {sigma}3 Structure J. Virol., August 28, 2002; 76(19): 9832 - 9843. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Chandran, D. L. Farsetta, and M. L. Nibert Strategy for Nonenveloped Virus Entry: a Hydrophobic Conformer of the Reovirus Membrane Penetration Protein {micro}1 Mediates Membrane Disruption J. Virol., August 28, 2002; 76(19): 9920 - 9933. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. H. Ebert, J. Deussing, C. Peters, and T. S. Dermody Cathepsin L and Cathepsin B Mediate Reovirus Disassembly in Murine Fibroblast Cells J. Biol. Chem., June 28, 2002; 277(27): 24609 - 24617. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Golden, J. Linke, S. Schmechel, K. Thoemke, and L. A. Schiff Addition of Exogenous Protease Facilitates Reovirus Infection in Many Restrictive Cells J. Virol., June 27, 2002; 76(15): 7430 - 7443. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Jane-Valbuena, L. A. Breun, L. A. Schiff, and M. L. Nibert Sites and Determinants of Early Cleavages in the Proteolytic Processing Pathway of Reovirus Surface Protein {sigma}3 J. Virol., April 16, 2002; 76(10): 5184 - 5197. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. L. Nason, J. D. Wetzel, S. K. Mukherjee, E. S. Barton, B. V. V. Prasad, and T. S. Dermody A Monoclonal Antibody Specific for Reovirus Outer-Capsid Protein {sigma}3 Inhibits {sigma}1-Mediated Hemagglutination by Steric Hindrance J. Virol., July 15, 2001; 75(14): 6625 - 6634. [Abstract] [Full Text]
|
|
|
|
|
|
K. Chandran, X. Zhang, N. H. Olson, S. B. Walker, J. D. Chappell, T. S. Dermody, T. S. Baker, and M. L. Nibert Complete In Vitro Assembly of the Reovirus Outer Capsid Produces Highly Infectious Particles Suitable for Genetic Studies of the Receptor-Binding Protein J. Virol., June 1, 2001; 75(11): 5335 - 5342. [Abstract] [Full Text]
|
|
|
|
|
|
D. H. Ebert, J. D. Wetzel, D. E. Brumbaugh, S. R. Chance, L. E. Stobie, G. S. Baer, and T. S. Dermody Adaptation of Reovirus to Growth in the Presence of Protease Inhibitor E64 Segregates with a Mutation in the Carboxy Terminus of Viral Outer-Capsid Protein {sigma}3 J. Virol., April 1, 2001; 75(7): 3197 - 3206. [Abstract] [Full Text]
|
|
|
|
|
|
G. S. Baer, D. H. Ebert, C. J. Chung, A. H. Erickson, and T. S. Dermody Mutant Cells Selected during Persistent Reovirus Infection Do Not Express Mature Cathepsin L and Do Not Support Reovirus Disassembly J. Virol., November 1, 1999; 73(11): 9532 - 9543. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Jané-Valbuena, M. L. Nibert, S. M. Spencer, S. B. Walker, T. S. Baker, Y. Chen, V. E. Centonze, and L. A. Schiff Reovirus Virion-Like Particles Obtained by Recoating Infectious Subvirion Particles with Baculovirus-Expressed sigma 3 Protein: an Approach for Analyzing sigma 3 Functions during Virus Entry J. Virol., April 1, 1999; 73(4): 2963 - 2973. [Abstract] [Full Text]
|
|
|
|
 |
|
 W. Canning and B. Fields Ammonium chloride prevents lytic growth of reovirus and helps to establish persistent infection in mouse L cells Science, February 25, 1983; 219(4587): 987 - 988. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
