HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Schultz, M. Articles by Crowell, R. L. Search for Related Content PubMed PubMed Citation Articles by Schultz, M. Articles by Crowell, R. L. Agricola Articles by Schultz, M. Articles by Crowell, R. L.

Acquisition of Susceptibility to Coxsackievirus A2 by the Rat L₈ Cell Line During Myogenic Differentiation

Department of Microbiology and Immunology, Langbord Virus Laboratory, Hahnemann Medical College, Philadelphia, Pennsylvania 19102, U.S.A.

Coxsackievirus A2 was propagated to high titres in the established rat L₈ myogenic cell line during the stage of differentiation characterized by fusion of myoblasts into multinucleated myotubes (192 h post-plating cultures). In contrast, pre-fusion mononucleated L₈ cultures infected 24 h after plating were refractory to Coxsackievirus A2 infection, as was a non-fusing clonal variant of the L₈ line (L₈CL3-U) regardless of the age of the latter cultures. The development of virus susceptibility in the older L₈ cultures correlated with a marked virus-specific c.p.e. as evidenced by vacuolated degenerating myotubes with disrupted cytoplasm, whereas no c.p.e. was seen following infection of the young L₈ or variant L₈CL3-U cultures. The restriction of Coxsackievirus A2 replication in young L₈ and L₈CL3-U cultures occurred after virus attachment.

Received 2 July 1979; accepted 26 July 1979.