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Comparison of Foldback Sequences of Herpes Simplex Virus Types 1 and 2 DNA

Department of Microbiology and Specialized Cancer Research Center, The Pennsylvania State University, College of Medicine, Hershey, Pennsylvania 17033, U.S.A.

The DNAs of herpes simplex virus types 1 and 2 (HSV-1 and HSV-2) were separately denatured and allowed to renature briefly. The intrastrand foldback structures that resulted from base pairing of inverted repeated sequences on otherwise single-stranded (ss) DNA were visualized in the electron microscope. The two genomes were found to contain similar size classes of small duplex stem DNA sequences. However, HSV-2 DNA appeared to possess an additional, larger size class of foldback structures not found on HSV-1 DNA. Both HSV DNAs were found to contain stem-plus-loop structures; the larger stem-plus-loop structures of the two genomes had similar stem lengths but dissimilar loop lengths. Thus, a comparison of the genomes of HSV-1 and HSV-2 showed that they possessed similar size classes of foldback sequences.

^* Author to whom correspondence should be addressed.

Received 12 May 1979; accepted 27 July 1979.