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Immunoglobulins in Human Serum Reactive with Murine Friend Leukaemia Virus

Institute of Microbiology, University of Pisa, Via S. Zeno, 39 Pisa
¹ Laboratory of Cell Biology, Tumour Immunobiology Unit, C.N.R., Via Romagnosi 18/A, Rome, Italy

Fresh human sera neutralize Friend leukaemia virus (FLV). The activity is abolished by heating (56 °C for 30 min), hydrazine and EDTA treatment, suggesting the involvement of complement. Human sera also contain antibody which specifically reacts with FLV. Following incubation with human whole serum, IgG and F(ab')₂ fragments, FLV acquire the ability to bind anti-human Ig sera, as shown in neutralization and radioimmunoprecipitation assays. The same is true for FLV-infected cells but not for uninfected control cells, as assessed by immunofluorescence. The binding of antibody to FLV-infected cells is prevented by pre-treating the cells with antisera to FLV and FLV-gp71, and is competed by FLV, FLV-gp71 and, to a lesser extent, by FLV-p12 and p30.

^* To whom reprint requests should be addressed.

Received 17 April 1979; accepted 3 September 1979.