HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Lanser, J. A. Articles by Howard, C. R. Search for Related Content PubMed PubMed Citation Articles by Lanser, J. A. Articles by Howard, C. R. Agricola Articles by Lanser, J. A. Articles by Howard, C. R.

The Polypeptides of Infectious Bronchitis Virus (IBV-41 Strain)

Colin R. Howard

Department of Medical Microbiology, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT

The Massachusetts strain of avian infectious bronchitis virus was purified from embryonated hens' eggs. Four major species of apparent mol. wt. 90000, 52000, 29000 and 26000 were resolved by SDS-polyacrylamide gel electrophoresis. Omission of reducing agent failed to resolve the 29000 mol. wt. component. Labelling of acrylamide gels with ¹²⁵I-concanavalin A indicated that polypeptides of mol. wt. 90000, 29000 and 26000 were glycosylated and, in the absence of reducing agent, that the 29000 species migrated as a dimer in the 5000 mol. wt. region. Purified IBV radio-iodinated with Bolton and Hunter reagent, which banded as a single peak of radioactivity in Metrizamide gradients, was found to contain bands of radioactivity when analysed by SDS-PAGE, corresponding to the polypeptides of mol. wt. 90000, 52000 and 29000 resolved in stained gels. Disruption of IBV particles in Triton X-100 released two subviral particles, a 16 nm spike which comprised polypeptides of 90000, 52000 and 29000 mol. wt. and another denser spherical particle of 25 to 45 nm which contained RNA and the 52000 and 26000 polypeptides.

^* Present address: Department of Medical Microbiology, Flinders Medical Centre, Bedford Park, South Australia 5042.

To whom reprint requests should be addressed.

Received 24 April 1979; accepted 5 September 1979.

This article has been cited by other articles:

				E. Corse and C. E. Machamer Infectious Bronchitis Virus E Protein Is Targeted to the Golgi Complex and Directs Release of Virus-Like Particles J. Virol., May 1, 2000; 74(9): 4319 - 4326. [Abstract] [Full Text]

				C. A. M. de Haan, L. Kuo, P. S. Masters, H. Vennema, and P. J. M. Rottier Coronavirus Particle Assembly: Primary Structure Requirements of the Membrane Protein J. Virol., August 1, 1998; 72(8): 6838 - 6850. [Abstract] [Full Text] [PDF]