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Electron Microscopic Immunoperoxidase Studies on the Accumulation of Virus Antigen in Cells Infected with Shope Fibroma Virus

Bernhard Nocht Institute for Nautical and Tropical Medicine, Department of Virology, Bernhard Nocht-Str. 74, 2000 Hamburg 4, West Germany

The indirect immunoperoxidase technique was used to investigate the development of the virus-specific intracellular and cell membrane antigens in cells infected with the Shope fibroma virus. Starting with 6 h p.i., virus antigen formed distinct inclusions within the cytoplasm frequently enclosed by endoplasmic reticulum. The endoplasmic reticulum disappeared almost completely 10 to 12 h p.i., coincidentally with the beginning of virus formation. The virus antigen was distributed throughout the cytoplasm. At the same time virus-induced antigen began to appear at the cell membrane and subsequently increased. No cytochemical staining could be observed on the endoplasmic reticulum, within the nucleus and within immature and mature virus particles. The correlation between antigen synthesis and changes in cell ultrastructure is discussed.

^* Present address: Heinrich Pette-Institute for Experimental Virology and Immunology at the University of Hamburg, 2000 Hamburg 20, Martinistr. 52, West Germany.

Received 19 July 1979; accepted 10 September 1979.