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Lack of Temperature Sensitivity of RNA-dependent RNA Polymerases Isolated from Tobacco Plants Infected with ts Mutants of Alfalfa Mosaic Virus

Department of Biochemistry State University of Leiden, P.O. Box 9505 2300 RA Leiden The Netherlands

Enzyme activities of particulate and solubilized RNA-dependent RNA polymerase preparations from tobacco plants infected with 10 ts mutants of alfalfa mosaic virus (AMV), having their mutations in each of the three parts of the virus genome, were found to behave similarly with respect to temperature as compared to preparations obtained from plants infected with wild type AMV or from uninfected plants. Thus it is very unlikely that a virus-specific protein is involved in these in vitro RNA polymerase activities.

Received 31 May 1979; accepted 10 September 1979.