HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Paul, H. L. Articles by Huth, W. Search for Related Content PubMed Articles by Paul, H. L. Articles by Huth, W. Agricola Articles by Paul, H. L. Articles by Huth, W.

Serological Studies on the Relationships of Some Isometric Viruses of Gramineae

Biologische Bundesanstalt für Land- und Forstwirtschaft, Institut für Viruskrankheiten der Pflanzen, Braunschweig, West Germany

Particles of 17 isolates of viruses from Gramineae were purified and the virus relationships assessed by different serological techniques. From the reaction end-points of antisera in agar gel double diffusion tests the serological differentiation indices of reciprocal tests were calculated. These indices were used to construct a scheme of serological interrelationship. Spur formation tests were used to distinguish between closely related, non-identical viruses. The isolates were shown to belong to four groups, one of which (the cocksfoot mild mosaicpanicum mosaic virus group) contains most of the isolates and can be subdivided into two main clusters (typified by cocksfoot mild mosaic and panicum mosaic viruses, respectively). Additional information was obtained by immunoelectrophoresis of the viruses with their homologous antisera and by the determination of the mol. wt. of the coat protein subunits using SDS-polyacrylamide gel electrophoresis. The data obtained by both methods agree with the above mentioned results, although some minor differences were found between the immunoelectrophoretic patterns of panicum mosaic virus and its relatives and also the three isolates of Cynosurus mottle virus. Reversed rocket electrophoresis did not provide any information of use in virus grouping. Brome mosaic virus did not react with the antisera to the 17 viruses, but tobacco mosaic virus reacted weakly with all the antisera and also with one of the antisera to brome mosaic virus. These and other recently published results are discussed.

Received 22 June 1979; accepted 5 October 1979.