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Detection of Avian Oncovirus Group-specific Antigens by the Enzyme-linked Immunosorbent Assay

Department of Microbiology, State University of New York, Upstate Medical Center, 766 Irving Avenue, Syracuse, New York 13210, U.S.A.

A three-step sandwich enzyme-linked immunosorbent assay was developed for the detection of avian oncovirus group-specific (gs) antigens. The assay procedure was to coat the wells of microtitre plates with hamster anti-gs IgG, react with crude or purified antigen and finally with hamster anti-gs IgG linked to horseradish peroxidase. The sensitivity was 8 picograms (pg) of input avian myeloblastosis virus (AMV) protein, with negligible background. As the ELISA takes less than 2 h to perform, large-scale screening for infected birds is feasible. A blocking assay was also developed for detecting anti-gs antibodies by adding unlabelled antiserum after the antigen step.

Received 24 August 1979; accepted 12 October 1979.