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Synthesis, Accumulation and Encapsidation of Individual Brome Mosaic Virus RNA Components in Barley Protoplasts

Department of Horticulture, University of Wisconsin, Madison, Wis. 53706, U.S.A.

The rates of virus RNA synthesis and virion accumulation were investigated in brome mosaic virus-infected barley protoplasts. Single-stranded virus RNAs could be detected as early as 6 h after inoculation. Only RNA components 1 and 2 were detected at this time, suggesting that their synthesis is initiated relatively early in infection. The RNAs were synthesized at similar rates from 16 to 35 h post inoculation with maximal synthesis until approx. 25 h after inoculation. Double-stranded replicative forms of the four virus RNAs were observed. Their synthesis was first detectable at 6 h post inoculation and followed a time course similar to that of the single-stranded RNA species. Analysis of RNA encapsidation and infectivity assays of protoplast homogenates revealed that virion formation was greatest between 10 and 25 h after inoculation. All four RNAs were present in virions at 10 h post inoculation. Particles containing RNA 3 and RNA 4 accumulated at a faster rate than particles containing RNA 1 or RNA 2.

Received 30 August 1979; accepted 25 October 1979.

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