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Preparative Isoelectric Focusing of Poliovirus Polypeptides in Urea-Sucrose Gradients

Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie an der Universität, Hamburg, Martinistrasse 52, 2000 Hamburg 20, Federal Republic of Germany

Isoelectric focusing in urea using small density gradient columns for dissociated poliovirus resulted in a complete separation of the four virus polypeptides. Identification and purity of VP1, 2, 3 and 4 was shown by SDS-polyacrylamide gel electrophoresis. The isoelectric points were determined and compared with the values obtained in gels. The recovery of the individual polypeptides was about 80%. Up to 500 µg of poliovirus per tube could thus be separated in a one-step procedure giving pure and SDS-free poliovirus polypeptides.

Received 10 August 1979; accepted 9 November 1979.