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Monoclonal Antibodies to Influenza Matrix Protein: Detection of Low Levels of Matrix Protein on Abortively Infected Cells

National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA

R. G. Webster and K. van Wyke

Division of Virology St Jude Childrens Research Hospital, 332 N. Lauderdale, P.O. Box 318 Memphis, Tenn. 38101, U.S.A.

We have quantified the appearance of matrix (M-) protein on P815 mastocytoma cells infected with type A influenza virus using a monoclonal antibody specific for M-protein. In contrast to previously reported values, only low amounts (about 10³ molecules/cell) of M-protein appear on the cell surface up to 16 h p.i. Since P815 cells are excellent targets for cross-reactive T-cell lysis 3 to 5 h after virus infection, when only about 10² M-protein sites are found on each cell surface, it appears less likely that the recognition of M-protein can account for the cross-reactivity of cytotoxic T-lymphocytes for different type A influenza viruses.

Received 17 September 1979; accepted 2 November 1979.

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