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J Gen Virol 48 (1980), 105-109; DOI 10.1099/0022-1317-48-1-105
© 1980 Society for General Microbiology

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Use of Hybridoma Monoclonal Antibodies in the Detection of Antigenic Differences Between Rabies and Rabies-related Virus Proteins. II. The Glycoprotein

A. Flamand*, T. J. Wiktor{dagger} and H. Koprowski

The Wistar Institute of Anatomy and Biology, 36th Street at Spruce, Philadelphia, Pennsylvania 19104, U.S.A.

Twenty-five hybridoma cultures secreted monoclonal antibodies directed against the glycoprotein of rabies or rabies-related viruses. The antibodies had different specificities for the glycoproteins of eight rabies and rabies-related viruses. They could be classified into fourteen groups which probably correspond to different antigenic determinants on the glycoproteins. These hybridomas when used in either radioimmunoassay (RIA) or in neutralization tests allow differentiation of laboratory strains of rabies virus from each other as well as from the rabies-related viruses.

* On leave from Centre National de la Recherche Scientifique, Université Paris Sud, Orsay, France.

{dagger} To whom reprint requests should be addressed.

Received 25 October 1979; accepted 14 December 1979.


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