HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Lake, J. R. Articles by Cooper, P. D. Search for Related Content PubMed PubMed Citation Articles by Lake, J. R. Articles by Cooper, P. D. Agricola Articles by Lake, J. R. Articles by Cooper, P. D.

Deletions of the Terminal Sequences in the Genomes of the White Pock (u) and Host-restricted (p) Mutants of Rabbitpox Virus

P. D. Cooper

Department of Microbiology, John Curtin School of Medical Research, Australian National University, P.O. Box 334, Canberra City, A.C.T., Australia 2601

The DNAs of wild-type rabbitpox virus (u⁺.p⁺), and selected u (white pock, u.p⁺) and p (white pock, PK-15 cell non-permissive, u.p.) mutants were compared by restriction enzyme analysis. The cleavage fragments produced by digestion with HindIII or EcoR1 endonucleases were separated by agarose gel electrophoresis. Deletions of approx. 10 x 10⁶ were recognized at the right-hand terminus of each u mutant (three isolates), whereas deletions of 5 x 10⁶ to 20 x 10⁶ were detected at the left-hand terminus of each p mutant (nine isolates). Rapidly renaturing terminal restriction fragments, indicative of the covalent cross-links, were only found at the terminus unaffected by deleted sequences. Recombinant viruses that were wild-type in both genetic characters and in restriction pattern were recovered from mixed infections involving u and p mutants, but no genetic interaction was detected between crosses involving only u or p mutants. The p mutants were divided into two non-permissive classes dependent on their biochemical expression in PK-15 cells: the ‘early’ class which failed to replicate virus DNA and synthesized only the ‘early’ polypeptides and the ‘late’ class which appeared to be normal both in virus DNA and polypeptide synthesis. There was no correlation between the extent of the left-hand terminal deletion and the extent of permissiveness of the p mutants in PK-15 cells.

^* Present address: Department of Medical Virology, Institute of Medical and Veterinary Science, Frome Road, Adelaide, South Australia 5000.

To whom reprint requests should be addressed.

Received 27 July 1979; accepted 17 December 1979.