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A Comparison of Measles and Canine Distemper Virus Polypeptides

Department of Biochemistry, The Queen's University, Belfast BT9 7BL, N., Ireland

The polypeptides induced by canine distemper virus (CDV) strains have been characterized by polyacrylamide slab gel electrophoresis of infected cell lysates labelled with ³⁵S-methionine, ¹⁴C-amino acids, ³H-glucosamine and ³H-mannose, or ³²P-orthophosphate. Seven virus-induced polypeptides have been assigned the following nomenclature and mol. wt.: a large polypeptide L (180000); a large glycoprotein H (77000); a nucleocapsid-associated protein P (73000); the nucleocapsid protein N (60000); the smaller glycoprotein F₀ (59000); a membrane protein M (35000) and a small polypeptide S (15000). During pulse-chase experiments with ³H-glucosamine and ¹⁴C-amino acids the intensity of the F₀ band decreases and that of the F₁ and F₂ bands increases; the H polypeptide band becomes more diffuse and the S-protein disappears. The N- and P- but not the M-proteins have been found to be phosphorylated. The polypeptide pattern of the Onderstepoort strain of CDV has been compared with that of two other CDV and with 17 measles and subacute sclerosing panencephalitis (SSPE) strains. Differences in the mobilities of various polypeptides have been observed between CDV and measles and SSPE strains; however, the only consistent difference is the mol. wt. of the M-protein of CDV strains which is smaller by 2000 than that of MV and this may be a biochemical marker to distinguish CDV from measles and SSPE virus strains.

^* Present address: The James A. Baker Institute for Animal Health, Cornell University, Ithaca, N.Y. 14053, U.S.A.

Received 21 August 1979; accepted 17 December 1979.