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The Polypeptide Composition of Isolated Surface Projections of Avian Infectious Bronchitis Virus

Poultry Department Central Veterinary Laboratory, New Haw, Weybridge, Surrey KT15 3NB, U.K.

Disruption of avian infectious bronchitis virus (IBV) particles with 4% Triton X-100 and 1.0 M-KCl and centrifugation through a sucrose gradient containing 0.1% Triton X-100 and 1.0 M-KCl enabled separation of the petal-shaped surface projections. By negative-contrast electron microscopy the separated projections appeared mainly as rosettes containing 3 to 12 projections radiating from a central core, although single projections and rosettes containing up to 16 projections were seen. SDS-PAGE of these preparations revealed two polypeptides of 86000 and 66000 mol. wt. The larger polypeptide was glycosylated.

Received 24 August 1979; accepted 20 November 1979.