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Catalysis of Adenovirus DNA Synthesis in vitro by DNA Polymerase

D. M. Rekosh

Division of Virology National Institute for Medical Research, Mill Hill, London NW7 1AA

DNA replication complexes were purified from adenovirus type 5 (Ad5)-infected HeLa cells. DNA synthesis by these complexes in vitro was extremely sensitive to the inhibitors dideoxythymidine triphosphate, N-ethyl maleimide and p-hydroxymercuribenzoate. The bound DNA polymerase was released from the complexes by limited digestion with micrococcal nuclease. This released polymerase preferred poly(rA):(dT)_12–18 as template over activated calf thymus DNA. These results are compatible with the major polymerase in the replication complex being of the class.

^* Present address: Max-Planck-Institut fur Zuchtungsforschung, 5 Koln-Vogelsang, D5000 Koln 30, Germany.

Present address: Department of Biochemistry, Schools of Medicine and Dentistry, State University of New York at Buffalo, 102 Cary Hall, Buffalo, New York 14214, U.S.A.

Received 8 October 1979; accepted 26 November 1979.