HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Ghabrial, S. A. Articles by Shepherd, R. J. Search for Related Content PubMed Articles by Ghabrial, S. A. Articles by Shepherd, R. J. Agricola Articles by Ghabrial, S. A. Articles by Shepherd, R. J.

A Sensitive Radioimmunosorbent Assay for the Detection of Plant Viruses

Plant Pathology Department, University of Kentucky, Lexington, Kentucky 40546 and Department of Plant Pathology, University of California, Davis, California 95616, U.S.A.

A simple and highly sensitive radioimmunosorbent assay (RISA) for the detection of plant viruses is described. The RISA procedure is a microplate method based on the principle of ‘double-antibody sandwich’ and follows essentially the protocol of the enzyme-linked immunosorbent assay (ELISA) (Clark & Adams, 1977), with the exception that ¹²⁵I-labelled -globulin is substituted for the -globulin enzyme conjugate; the bound ¹²⁵I--globulin is dissociated by acidification from the double-antibody sandwich. The radioactivity is proportional to virus concentration, and cauliflower mosaic virus (CaMV) and lettuce mosaic virus (LMV) could be detected at concentrations as low as 5 and 2 ng/ml, respectively. Direct evidence of the adverse effects of conjugation with enzyme on the binding abilities of antibodies is presented. The RISA procedure should prove valuable with viruses for which the ELISA values are too low to be dependable.

Received 12 October 1979; accepted 7 January 1980.