HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Furusawa, I. Articles by Kunoh, H. Search for Related Content PubMed Articles by Furusawa, I. Articles by Kunoh, H. Agricola Articles by Furusawa, I. Articles by Kunoh, H.

Infection of Turnip Protoplasts with Cauliflower Mosaic Virus

Laboratory of Plant Pathology Faculty of Agriculture Kyoto University, Kyoto 606, Japan

Mitsuru Kohno and Hitoshi Kunoh

Laboratory of Plant Pathology Faculty of Agriculture Mie University, Mie 514, Japan

Infection of turnip protoplasts with cauliflower mosaic virus (CaMV) was investigated using an immunofluorescent technique. Up to 80% of protoplasts were infected when they were inoculated with 10 µg/ml CaMV, together with 1 µg/ml poly-L-ornithine (PLO) in 20 mM-citrate buffer, pH 5.0. Infection was first detected by fluorescent antibody staining 24 h after inoculation and the CaMV inclusion bodies were observed by electron microscopy of the cytoplasm 72 h after inoculation.

Received 22 October 1979; accepted 7 January 1980.