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Human Cytomegalovirus DNA: Physical Maps for the Restriction Endonucleases BglII, HindIII and XbaI

Laboratorium voor de Gezondheidsleer, Universiteit van Amsterdam, Mauritskade 57, 1092 AD Amsterdam, The Netherlands

It is proposed that the genome of human cytomegalovirus (HCMV) consists of two unique sequences, L and S, bounded by two sets of redundant sequences (P. Sheldrick et al. unpublished data). In this arrangement the terminal sequences (TR₁ and TR_s) are repeated in an internal inverted form (IR₁ and IR_s) and delimit L and S. After restriction endonuclease cleavage of the DNA, four 0.5 M and four 0.25 M fragments are found, indicating that HCMV DNA preparations consist of four equimolar populations differing only in the relative orientation of the L and S components. Cleavage of the CMV DNA with the restriction endonucleases BglII, HindIII and XbaI results in 32, 27 and 21 fragments, respectively. The arrangement of these fragments has been determined using molecular hybridization techniques, identification of terminal fragments and the identification of linkage groups by double-digestion. In this report the physical maps for the restriction endonucleases BglII, HindIII and XbaI are presented.

Received 10 October 1979; accepted 10 January 1980.

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