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J Gen Virol 49 (1980), 231-234; DOI 10.1099/0022-1317-49-1-231
© 1980 Society for General Microbiology
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Large Scale Production of Mouse Interferons from Monolayers of Ehrlich Ascites Tumour Cells

H. Taira, R. J. Broeze, E. Slattery and P. Lengyel

Department of Molecular Biophysics and Biochemistry Yale University, New Haven Connecticut 06511, U.S.A.

Conditions are described for the large scale production of mouse interferons from Ehrlich ascites tumour cells cultured as monolayers in roller bottles. With the procedure reported here, we have used 50 to 65 600 cm2 roller bottles to produce routinely 2 x 109 to 3 x 109 International units of crude mouse interferon/week with a specific activity of 1 x 106 to 1.5 x 106 units/mg protein.

Received 8 January 1980; accepted 7 February 1980.




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