HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by van Berlo, M. F. Articles by van der Zeijst, B. A. M. Search for Related Content PubMed PubMed Citation Articles by van Berlo, M. F. Articles by van der Zeijst, B. A. M. Agricola Articles by van Berlo, M. F. Articles by van der Zeijst, B. A. M.

Temperature-sensitive Mutants of Equine Arteritis Virus

Institute of Virology, Veterinary Faculty, State University, Utrecht, Yalelaan 1, The Netherlands

Seventeen temperature-sensitive mutants of equine arteritis virus, a nonarthropod-borne togavirus, have been isolated. 5-Fluorouracil, 0-methylhydroxylamine and ethyl methanesulphonate were used as mutagens. The mutants were characterized by their ability to synthesize virus RNA and virus proteins at the permissive (35 °C) and restrictive temperature (40 °C) using autoradiography of cells labelled with ³H-uridine in the presence of actinomycin D and immunofluorescence respectively. Among the mutants, four were unable to synthesize virus RNA and virus proteins at 40 °C (RNA^-/protein^-). The other mutants were RNA^-/protein⁺ (3); RNA^±/protein^- (2); RNA⁺/protein⁺ (6) and RNA⁺/protein^- (1).

Received 12 November 1979; accepted 8 February 1980.