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Electron Microscope Unit and Department of Microbiology John Curtin School of Medical Research, Australian National University Canberra, A.C.T. Australia, 2600
Purified suspensions of Sericesthis iridescent virus were treated with a nasal decongestant, negatively stained and examined in the electron microscope. The outer icosahedral surface of the virus particles showed morphological subunits apparently in close-packed hexagonal array and 70 Å apart. The orientation of untreated particles was determined from their outline. This made it possible to measure precisely their icosahedral edge-length (860 ± 27 Å).
Prolonged storage of purified virus in distilled water at 4° resulted in disintegration of the particles into triangular, pentagonal and linear fragments. The triangles of side 700 Å were clearly composed of 55 hexagonally arrayed subunits 70 Å apart. Edges of the pentagons appeared to consist of three subunits also about 70 Å apart, while the linear fragments had a broad length distribution about a mean of 438 Å.
All these observations, interpreted by a new approach to the ‘Goldberg diagram’, suggested that the virus surface is composed of 1562 morphological subunits, though alternatives of 1292 and 1472 subunits cannot be excluded.
Received 30 December 1968;
accepted 17 March 1969.
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