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Arbovirus Plaquing in Two Simian Kidney Cell Lines

Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, Connecticut

A total of 212 arbovirus serotypes, representing 29 arbovirus serogroups, were screened for their plaque-forming ability and titrated in two continuous simian kidney cell lines (VERO and LLC-MK₂). Plaquing data were obtained with 205 viruses in either one or both of these cell lines; seven viruses failed to plaque in either line. Both cell lines were susceptible to 169 arbovirus serotypes. Seventeen viruses failed to plaque in VERO and 18 in LLC-MK₂. Plaques appeared as early as 2 days, and as late as 18 days (Triniti virus in VERO) Plaque diameters ranged from 1 to 28 mm. during a 24-day observation period.

Received 11 March 1969; accepted 28 April 1968.

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