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Gel Filtration on Agarose in the Separation of Adeno-associated Viruses from Adenoviruses and Their Structural Components

Department of Research and Development Wyeth Laboratories, Inc. Philadelphia, Pennsylvania 19101, U.S.A.

Gel filtration on agarose (agar) has been used to separate viruses differing in size (Bengtsson & Philipson, 1964; ech, 1962; Fridborg et al. 1965; Steere & Ackers, 1962). We therefore tried it for the separation of adeno-associated viruses (AAV) from adenoviruses, including the structural components of the latter. The results presented here indicate that chromatography on Sepharose 2B (Pharmacia, Uppsala, Sweden) represents a useful step for such separation.

The source of AAV and adenovirus was similar to that used in previous studies (Hoggan, Blacklow & Rowe, 1966) with adenovirus type 7, strain LLE46⁺, containing the adenovirus SV40 hybrid and AAV type 1. The viruses were grown in African green monkey kidney cells. The preparation of [³H]thymidine labelled adenovirus type 7 and the measurement of radioactivity are described in a subsequent paper (A. R. Neurath, B. A. Rubin & R. W. Hartzell, 1969, in preparation).

Received 30 April 1969; accepted 23 June 1969.