HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Bastin, M. Articles by Bourgaux, P. Search for Related Content PubMed PubMed Citation Articles by Bastin, M. Articles by Bourgaux, P. Agricola Articles by Bastin, M. Articles by Bourgaux, P.

Biological Properties of Polyoma DNA Fragments Cloned in Plasmid pBR322

Département de microbiologie Centre hospitalier universitaire, Sherbrooke, Québec, Canada

The two HindIII fragments of polyoma virus DNA were cloned in the HindIII site of plasmid pBR322, and the biological activity of the recombinant plasmids was tested in tissue culture cells. A mixture of recombinant plasmids containing the HindIII-A and HindIII-B fragments was infectious, but only after cleavage with HindIII. Recombinant plasmids containing the HindIII-A fragment, but not those containing the HindIII-B fragment, induced the transformation of Fischer rat 3T3 cells. These findings indicate that about half of the early region of polyoma virus DNA is not essential for the initiation or the maintenance of transformation.

Received 22 January 1980; accepted 6 March 1980.

This article has been cited by other articles:

				S. Slilaty and H. Aposhian Gene transfer by polyoma-like particles assembled in a cell-free system Science, May 13, 1983; 220(4598): 725 - 727. [Abstract] [PDF]