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J Gen Virol 50 (1980), 185-190; DOI 10.1099/0022-1317-50-1-185
© 1980 Society for General Microbiology
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Plaque Formation and Purification of BK Virus in Cultured Human Urinary Cells

George H. Sack, Jun., Jeanette S. Felix and Anthony A. Lanahan

The Johns Hopkins University School of Medicine Departments of Pediatrics and Medicine Baltimore, Md. 21205, U.S.A.

Primary human urinary cells support growth and plaque formation by papovavirus BK, permitting plaque purification of the virus. After plaquing, the virus forms uniform plaques and its DNA has a homogeneous restriction enzyme fragment profile. Support of BK replication as well as morphological and cultural characteristics suggest an epithelial origin for the cells.

Received 18 February 1980; accepted 10 March 1980.




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