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J Gen Virol 50 (1980), 195-200; DOI 10.1099/0022-1317-50-1-195
© 1980 Society for General Microbiology
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Investigation of Immunoperoxidase-labelled Rotavirus in Tissue Culture by Light and Electron Microscopy

D. Chasey

Central Veterinary Laboratory New Haw, Weybridge Surrey KT15 3NB, U.K.

A tissue culture-adapted strain of bovine rotavirus, grown in calf kidney monolayers, has been examined by light and electron microscopy after immunoperoxidase labelling. Some of the characteristic problems associated with pre-embedding methods have been demonstrated. Preparative techniques involving pretreatment of infected cells with a detergent followed by a detergent/fixative combination have enabled virus antigen to be labelled while maintaining satisfactory ultrastructural preservation.

Received 6 December 1979; accepted 1 April 1980.




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