J Gen Virol
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J Gen Virol 50 (1980), 59-68; DOI 10.1099/0022-1317-50-1-59
© 1980 Society for General Microbiology
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Detection of IgM Antibodies to Cytomegalovirus (CMV) Using an Enzyme-labelled Antigen (ELA)

H. Schmitz, U. von Deimling and B. Flehmig1

Abteilung für Virologie, Bernhard-Nocht-Institut für Schiffs- und Tropenkrankheiten, Bernhard-Nocht-Str. 74, 2000 Hamburg, 4, West Germany
1 Abteilung für Medizinische Virologie, Hygiene-Institut der Universität, Tübingen, West Germany

We have applied a peroxidase enzyme-labelled antigen (ELA) for the detection of IgM antibodies to cytomegalovirus: microtitre plates were coated with anti-IgM immunoglobulin. The IgM fraction of human serum was selectively bound to the precoated plates and the virus-specific IgM antibody was then detected by the enzyme-labelled antigen. A very efficient technique for the labelling of virus antigen is described. The IgM antibody was detected simply and specifically, Rheumatoid factor IgM did not interfere with this test.

Received 31 January 1980; accepted 2 April 1980.


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