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DNA-binding Proteins in the Nuclei of Herpes Simplex Virus-infected, Arginine-deprived, BSC-1 Cells

Department of Molecular Virology, The Hebrew University — Hadassah Medical School, Jerusalem, Israel

Nine proteins accumulate in nuclei of arginine-deprived herpes simplex virus-infected cells. Of these, eight were found to bind to double-stranded DNA-cellulose and to elute with 0·3 and 0·6 M-KCl, while one of 160000 mol. wt. had no affinity for the DNA. Five of the DNA-binding proteins were identified as virus enzymes: two were DNA polymerases, two were alkaline DNases and one was the thymidine kinase.

^* Present address: Ames-Yissum Ltd., Jerusalem, Israel.

Received 6 March 1980; accepted 2 May 1980.