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Characterization of Two Morphology Mutants of Autographa californica Nuclear Polyhedrosis Virus with Large Cuboidal Inclusion Bodies

Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada K7L 3N6

Two morphology mutants designated m-5 and m-6, of Autographa californica nuclear polyhedrosis virus (NPV) were isolated from virus grown in the presence of N-methyl-N'-nitro-N-nitrosoguanidine. Infected cells contained single, large cuboidal inclusion bodies with a crystalline lattice ultrastructure and envelope similar to that of wild-type (wt) polyhedra. The inclusion bodies had low infectivity when fed to Trichoplusia ni larvae. The paracrystalline lattice structure of the mutants was similar to wt, but occluded viruses were rarely found within the mutants. The m-5 polyhedrin (mol. wt. 30000) could be distinguished from wt and m-6 polyhedrin on the basis of migration in SDS-PAGE gels. Peptide maps of polyhedrin were obtained following digestion with chymotrypsinogen or Staphylococcus aureus V8 protease. They were identical for m-6 and wt polyhedrin but m-5 polyhedrin gave a different pattern. Thus, the altered morphology may be due to a change in polyhedrin composition not detectable in m-6 polyhedrin by the methods used here, or it may be the result of a mutation affecting a protein not yet identified.

^* Present Address: Département de Microbiologie, Centre Hospitalier Universitaire, Université de Sherbrooke, Sherbrooke, Québec, Canada J1H 5N4.

Received 4 February 1980; accepted 15 May 1980.

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